CST, 49349S, Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Myosin IIa. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody (CST #49349) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57863 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total myosin IIa protein. This antibody does not cross-react with myosin IIb or myosin IIc protein. This antibody cross-reacts with 20-25 kDa bands of unknown origin by western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1954 of human myosin IIa protein. Background Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, , , and , which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). Alternate Names BDPLT6; Cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MGC104539; MHA; MYH9; MYH9 variant protein; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Myosin-9; myosin, heavy chain 9, non-muscle; NMHC-II-A; NMMHC II-a; NMMHC-A; NMMHC-IIA; NMMHCA; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG