CST, 49381T, Pyruvate Carboxylase (E6S3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Pyruvate Carboxylase. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Pyruvate Carboxylase (E6S3A) Rabbit Monoclonal Antibody (CST #49381) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Pyruvate Carboxylase (E6S3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total pyruvate carboxylase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human pyruvate carboxylase protein. Background Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate in an anaplerotic pathway to replenish TCA cycle intermediates. It is also critical in regulating gluconeogenesis in the liver (1). Studies show that the increase of PC flux contributes to the induction of hepatic gluconeogenesis by glucagon (2). In addition, high-fat diet is shown to promote hepatic glucose uptake and induce PC activity in the liver (3). Loss of hepatic PC prevents diet-induced hyperglycemia and insulin resistance. Furthermore, this enzyme is also required for antioxidant defenses (1). Alternate Names PC; PCB; PYC; pyruvate carboxylase; Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG