CST, 49389S, LINGO-1 Antibody

Polyclonal Antibody for studying LINGO1. Validated for Western Blotting. Highly specific and rigorously validated in-house, LINGO-1 Antibody (CST #49389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LINGO-1 Antibody recognizes endogenous levels of total LINGO-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu400 of human LINGO-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Leucine-rich repeat and immunoglobulin domain-containing protein 1 (LINGO-1) is a potent negative modulator of neuronal processes, including neuronal survival, axonal integrity, oligodendrocyte differentiation, and myelination (1-5). LINGO-1, Nogo receptor (NgR), and p75 neurotrophin receptor (p75NTR), or TNF receptor orphan Y (TROY) form a tripartite receptor complex, which activates RhoA/ROCK signaling and is responsible for the inhibition effect of myelin- associated factors (6,7). LINGO-1 is abundantly expressed in the brain and is implicated in various neurodegenerative disorders, such as Essential tremor, multiple sclerosis, and Parkinson's disease (8-11). Recently, LINGO-1 was reported to bind directly to amyloid precursor protein (APP), promoting its degradation through lysosomal proteolysis (12). This research study implicated that LINGO-1 plays a critical role in the pathophysiology of Alzheimer's disease. Alternate Names FLJ14594; LERN1; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat and immunoglobulin domain-containing protein 1; Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1; leucine-rich repeat neuronal 6A; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LIGO1; LINGO1; LRRN6A; MGC17422; MRT64; UNQ201 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 98 SOURCE: Rabbit