CST, 4939T, Axl (C2B12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Axl. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Axl (C2B12) Rabbit Monoclonal Antibody (CST #4939) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Axl (C2B12) Rabbit Monoclonal Antibody detects endogenous levels of Axl protein. Species Reactivity: Human, Monkey, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human Axl proteins. Background Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4). Alternate Names ARK; AXL; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; JTK11; oncogene AXL; Tyro7; Tyrosine-protein kinase receptor UFO; UFO Specification REACTIVITY: H Mk Pg SENSITIVITY: Endogenous MW (kDa): 138 Source/Isotype: Rabbit IgG