CST, 49401S, HMGB3 (F2P1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HMGB3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HMGB3 (F2P1J) Rabbit Monoclonal Antibody (CST #49401) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HMGB3 (F2P1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMGB3 protein. This antibody does not recognize HMGB1 or HMGB2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly164 of human HMGB3 protein. Background High mobility group protein B3 (HMGB3), also known as HMG2A or HMG4, belongs to a family of highly conserved proteins that contain HMG box domains (1,2). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (2). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (3). HMGB3 is only expressed during embryogenesis (4). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix (1,2). HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA-binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (1,2). HMGB3 plays a major role in hematopoietic stem cells, striking a balance between self-renewal and differentiation (5-7). It has also been shown to be upregulated in a wide variety of tumor types, including non-small cell lung, bladder, and esophageal cancers (8-10). Alternate Names high mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group box 3; HMG-2a; HMG-4; HMG2A; HMG4; HMGB3; MGC90319; non-histone chromosomal protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG