CST, 49426T, CENP-I (D4D5K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CENPI. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CENP-I (D4D5K) Rabbit Monoclonal Antibody (CST #49426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CENP-I (D4D5K) Rabbit Monoclonal Antibody recognizes endogenous levels of total CENP-I protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn41 of human CENP-I protein. Background Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1). CENP-I is a centromere protein associated with the inner kinetochore, and required for progression through mitosis and the appropriate localization of other kinetochore-associated and checkpoint proteins, including CENP-C, the RZZ complex, MAD1 and MAD2 (2-4). Alternate Names CENP-I; CENPI; Centromere protein I; Follicle-stimulating hormone primary response protein; FSH primary response 1; FSH primary response protein 1; FSHPRH1; ICEN19; Interphase centromere complex protein 19; Leucine-rich primary response protein 1; LRPR1; Mis6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG