CST, 4942S, PBK/TOPK Antibody

Polyclonal Antibody for studying PBK/TOPK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PBK/TOPK Antibody (CST #4942) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PBK/TOPK Antibody detects endogenous levels of total PBK/TOPK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the carboxy-terminus of human PBK/TOPK. Antibodies are purified by protein A and peptide affinity chromatography. Background PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-binding domain, which is thought to interact with the human homologue of the Drosophila Discs large (hDlg) tumor suppressor protein (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclin B has been shown to phosphorylate PBK/TOPK , presumably at Thr9 (5). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4). Alternate Names Cancer/testis antigen 84; CT84; epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PBK; PDZ binding kinase; PDZ-binding kinase; serine/threonine protein kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit