CST, 49449SF, CYP17A1 (E6A7G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CYP17A1. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, CYP17A1 (E6A7G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49449) is ready to ship. Product Usage Information This product is the carrier free version of product #94004. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 94004 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CYP17A1 (E6A7G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CYP17A1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val377 of human CYP17A1 protein. Background CYP17A1, also known as cytochrome P450C17, is a steroidogenic enzyme belonging to the P450 cytochrome superfamily of monooxygenases (1, 2). In humans, CYP17A1 expression is abundantly expressed in the adrenal cortex, where it plays a central role in the androgen synthesis pathway (2). CYP17A1 is the primary target of abiraterone, a synthetic steroid used in the treatment of castration-resistant prostate cancer (CRPC) (3, 4). Abiraterone is converted to the more active form D4A, which antagonizes androgen receptor signaling by inhibiting CYP17A1 and other steroidogenic enzymes (3, 4). This suppresses the synthesis of 5α-dihydrotestosterone (DHT), which is a driver of castration-resistant prostate cancer cell growth (3, 4). Alternate Names 17-alpha-hydroxyprogesterone aldolase; CP17A; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; cytochrome P450 family 17 subfamily A member 1; cytochrome p450 XVIIA1; Cytochrome P450-C17; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; Cytochrome P450c17; P450C17; S17AH; Steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-monooxygenase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG