CST, 4944S, HR6A/HR6B Antibody

Polyclonal Antibody for studying UBE2A/UBE2B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HR6A/HR6B Antibody (CST #4944) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HR6A/HR6B Antibody detects endogenous levels of total HR6A and HR6B proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human HR6A. Antibodies are purified by protein A and peptide affinity chromatography. Background The ubiquitin-conjugating (UBC) enzymes HR6A and HR6B are the mammalian orthologues of the Saccharomyces cerevisiae Rad6 gene products (1). In S. cerevisiae, Rad6 facilitates cell cycle progression and ubiquitinates histone H2B (2,3). In vivo phosphorylation of HR6A Ser120 by cyclin-dependent kinases is thought to be important for the coordination and timing of ubiquitination events involved in cell cycle progression (4). In response to DNA damage, HR6A is known to interact physically with p53 and p14ARF, but knockout mice lacking HR6A or HR6B exhibit normal DNA damage responses (5,6). HR6B knockout males exhibit defective spermatogenesis, while HR6A knockout females fail to produce viable offspring (6). Alternate Names E2 protein; E2 ubiquitin-conjugating enzyme A; E2 ubiquitin-conjugating enzyme B; E2-17kDa; hHR6A; hHR6B; HR6A; HR6B; MRXS30; MRXSN; RAD6 homolog A; RAD6 homolog B; RAD6A; RAD6B; UBC2; UBE2A; UBE2B; Ubiquitin carrier protein A; Ubiquitin carrier protein B; ubiquitin conjugating enzyme E2 A; ubiquitin conjugating enzyme E2 B; ubiquitin conjugating enzyme E2B; Ubiquitin-conjugating enzyme E2 A; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2B (RAD6 homolog); Ubiquitin-protein ligase A; Ubiquitin-protein ligase B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 17 SOURCE: Rabbit