CST, 4946S, Phospho-WNK1 (Thr60) Antibody

Polyclonal Antibody for studying WNK1 (Thr60) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-WNK1 (Thr60) Antibody (CST #4946) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-WNK1 (Thr60) Antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 60. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresonding a region surrounding Thr60 of human WNK1. Antibodies are purified by protein A and peptide affinity chromatography. Background The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the and genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, which is thought to be required for its kinase activity (7). Alternate Names Erythrocyte 65 kDa protein; HSAN2; HSN2; hWNK1; KDP; KIAA0344; Kinase deficient protein; p65; PHA2C; PPP1R167; PRKWNK1; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; protein phosphatase 1, regulatory subunit 167; PSK; Serine/threonine-protein kinase WNK1; serine/threonine-protein kinase WNK1 1; serine/threonine-protein kinase WNK1 2; WNK lysine deficient protein kinase 1; WNK1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 230 SOURCE: Rabbit