CST, 49508SF, HLA-G (E8N9C) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HLA-G. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, HLA-G (E8N9C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49508) is ready to ship. Product Usage Information This product is the carrier free version of product #79769. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79769 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HLA-G (E8N9C) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HLA-G protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu102 of human HLA-G protein. Background HLA-G (HLA-G histocompatibility antigen, class I, G) is a non-classical MHC molecule expressed by trophoblasts in placenta, thymic epithelial cells, and some tumors. Alternative splicing leads to generation of at least six isoforms, four that are transmembrane proteins and two that are soluble (1-4). It is an inhibitory molecule involved in immune tolerance and escape, originally studied for its role in maternal tolerance of the fetus during pregnancy (1-5). HLA-G binds ILT2, ILT4, and KIR2DL4, playing a role in the regulation of natural killer, T, and monocyte/macrophage cells (4-5). Its involvement in evasion of immune response makes it a potential target for immunotherapy (6). Alternate Names b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G precursor; HLA G antigen; HLA-6.0; HLA-G; HLA-G histocompatibility antigen, class I, G; HLAG; major histocompatibility complex, class I, G; MHC class I antigen G; MHC class Ib antigen; MHC-G; mutant MHC class I antigen; mutant MHC class Ib antigen; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG