CST, 49509T, POU2F2 (F3B7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POU2F2. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, POU2F2 (F3B7M) Rabbit Monoclonal Antibody (CST #49509) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 47603 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity POU2F2 (F3B7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total POU2F2 protein. This antibody is predicted to detect multiple human and mouse POU2F2 isoforms. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POU2F2 protein. Background POU domain, class 2, transcription factor 2 (POU2F2), also known as Oct-2, is a transcription factor in the POU protein family (1-3). POU proteins contain two subunits: an N-terminal POU-specific domain and a C-terminal homeobox domain (1-3). Due to alternate splicing, POU2F2 has five human isoforms and seven mouse isoforms (3). POU2F2 is normally expressed in B cells and B cell lineage tumor cells, and is involved in B cell proliferation and differentiation by binding to immunoglobulin gene promoters (2-4). POU2F2 has also been shown to have a tumor-promoting role in some cancers, as well as potentially promoting oncogene-induced senescence (OIS) escape in early cancer progression (5,6). Alternate Names homeobox protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; OCT2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2; POU class 2 homeobox 2; POU domain, class 2, transcription factor 2; POU2F2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG