CST, 49553T, GPNMB (E7U1Z) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying GPNMB mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, GPNMB (E7U1Z) Mouse Chimeric Monoclonal Antibody (CST #49553) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity GPNMB (E7U1Z) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total GPNMB protein. This antibody does not cross-react with human GPNMB protein. Species Reactivity: Mouse Source / Purification This recombinant chimeric antibody is engineered from GPNMB (E7U1Z) Rabbit mAb #90205 according to animal-free protocols. This chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, GPNMB (E7U1Z) Rabbit mAb #90205, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse GPNMB protein. Background Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein overexpressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes (1), regulating osteoblast and osteoclast differentiation and function (2), stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells (3), enhancing autophagosome fusion to lysosomes in tissue repair, and regulating degradation of cellular debris (4,5). While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11). The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13). Alternate Names DC-H; DC-HIL; Dch; Dchil; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; glycoprotein (transmembrane) nmb; Gpnmb; Hgfin; ipd; iris pigment dispersion; Nmb; osteoactivin; Transmembrane glycoprotein NMB Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a