CST, 49622T, IL-2R gamma (E1V1O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL2RG. Validated for WB,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-2R gamma (E1V1O) Rabbit Monoclonal Antibody (CST #49622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:100 Flow Cytometry (Live): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity IL-2R gamma (E1V1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-2Rγ protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-2Rγ protein. Background IL-2Rγ (Cytokine receptor common subunit gamma, γc) is part of the interleukin-2 (IL-2) receptor, comprised of alpha, beta, and gamma chains (1-6). The gamma chain is required for the formation of high- and intermediate-affinity receptors, which consist of alpha beta gamma heterotrimers and beta gamma heterodimers (1). IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 all share the same common gamma chain, which was originally discovered as the gamma chain of the IL-2 receptor (3-6). The γ chain family of cytokines contribute to lymphocyte homeostasis by controlling aspects of lymphocyte development, growth, differentiation, and survival (5,6). In humans, a mutation in IL-2Rγ results in X-linked severe combined immunodeficiency (XSCID), which is a disease characterized by the absence of T and NK cells, with nonfunctional B cells (2). Alternate Names CD132; CD132 antigen; CIDX; common cytokine receptor gamma chain; common gamma-chain; Cytokine receptor common subunit gamma; gamma(c); gammaC; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; IL2RG; IMD4; interleukin 2 receptor subunit gamma; interleukin 2 receptor, gamma; interleukin 2 receptor, gamma (severe combined immunodeficiency); Interleukin-2 receptor subunit gamma; nonfunctional common cytokine receptor gamma chain; p64; SCIDX; SCIDX1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG