CST, 4969S, Endonuclease G Antibody

Polyclonal Antibody for studying EndoG. Validated for Western Blotting. Highly specific and rigorously validated in-house, Endonuclease G Antibody (CST #4969) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Endonuclease G Antibody detects endogenous levels of total Endonuclease G protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys122 of human Endonuclease G. Antibodies were purified by protein A and peptide affinity chromatography. Background Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation (1-5). EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria (1). During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process (3,6,7). Alternate Names Endo G; ENDOG; endonuclease G; Endonuclease G, mitochondrial; FLJ27463; mitochondrial endonuclease G; NUCG Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25, 30 SOURCE: Rabbit