CST, 49747T, DUSP3/VHR (F5R7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VHR/DUSP3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DUSP3/VHR (F5R7B) Rabbit Monoclonal Antibody (CST #49747) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DUSP3/VHR (F5R7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total DUSP3/VHR protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of DUSP3/VHR, but is not expected to detect other DUSP proteins. This antibody detects an approximately 25 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro162 of human DUSP3/VHR protein. Background Dual specificity protein phosphatase 3 (DUSP3), also known as Vaccinia H1-related phosphatase (VHR), is a small dual-specific phosphatase with specificity for MAP kinase ERK1/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen-stimulated T cells, DUSP3 is phosphorylated by Zap-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to downregulate the ERK and JNK pathways (3). Alternate Names dual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3; DUSP3; serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG