CST, 49761S, NINJ1 (F2Y6T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ninj1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NINJ1 (F2Y6T) Rabbit Monoclonal Antibody (CST #49761) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NINJ1 (F2Y6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NINJ1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length human NINJ1 protein. This antibody recognizes residues surrounding Ala42 of human NINJ1 protein. Background Ninjurin 1 (NINJ1) is a transmembrane protein initially identified for its involvement in nerve regeneration and cell adhesion (1). Research studies have also highlighted its primary function as a mediator of plasma membrane rupture (PMR) during various programmed cell death pathways, including pyroptosis, necroptosis, apoptosis, and ferroptosis, or in response to osmotic stress and mechanical tension (2). In response to death stimuli, NINJ1 oligomerizes and forms ring-like or filamentous structures on the plasma membrane, which lead to irreversible membrane damage, cell lysis, the release of damage-associated molecular patterns (DAMPs), and an inflammatory response in the extracellular environment (3-6). NINJ1 is also involved in leukocyte migration through facilitating cell-to-cell interactions between immune and endothelial cells, promoting the transendothelial migration of macrophages, and regulating Toll-like receptor 4 (TLR4) signaling (7,8). NINJ1-mediated transmigration of inflammatory cells across the blood-brain barrier contributes to neuroinflammation in experimental autoimmune encephalomyelitis (9), and NINJ1-mediated PMR of pyroptotic endothelial cells exacerbates blood-brain barrier destruction in traumatic brain injury (10). The role of NINJ1 in cancer biology is multifaceted and appears to be context-dependent, with evidence supporting both tumor-promoting (11-13) and tumor-suppressing functions (14-17). Alternate Names Nerve injury-induced protein 1; nerve injury-induced protein-1; NIN1; NINJ1; NINJURIN; ninjurin 1; Ninjurin-1; OTTHUMP00000021670 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG