CST, 4976T, Puma Antibody

Polyclonal Antibody for studying PUMA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Puma Antibody (CST #4976) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Puma Antibody detects endogenous levels of total Puma protein. This antibody also cross-reacts with an 18kDa band of unknown origin. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal region of human Puma. Antibodies are purified by protein A and peptide affinity chromatography. Background Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). Alternate Names BBC3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; BCL2 binding component 3; FLJ42994; JFY-1; JFY1; p53 up-regulated modulator of apoptosis; p53-upregulated modulator of apoptosis; PUMA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit