CST, 49830SF, Langerin (D9H7R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD207. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Langerin (D9H7R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49830) is ready to ship. Product Usage Information This product is the carrier free version of product #13650. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13650 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Langerin (D9H7R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Langerin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human Langerin protein. Background Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin, including Langerhans cells in the epidermis and langerin /CD103 dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8). Alternate Names C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; CD207; CD207 antigen, langerin; CD207 molecule; CD207 molecule, langerin; CLC4K; CLEC4K; Langerhans cell specific c-type lectin; Langerin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG