CST, 4983S, MAVS Antibody

Polyclonal Antibody for studying MAVS. Validated for WB,IP,IF,IF. Highly specific and rigorously validated in-house, MAVS Antibody (CST #4983) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MAVS Antibody detects endogenous levels of total MAVS/VISA protein. The bands detected at 52 and 75 kDa correlate with those described by Seth et al. (2005). Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys140 of mouse MAVS/VISA. Antibodies were purified by affinity chromatography. Background The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF, and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6). Alternate Names CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; Cardif; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IFN-B promoter stimulator 1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; IPS1; KIAA1271; MAVS; MGC3260; mitochondrial anti-viral signaling protein; mitochondrial antiviral signaling protein; Mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter variant 1b; virus-induced signaling adaptor; virus-induced signaling adaptor variant 1a; Virus-induced-signaling adapter; VISA Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 75, 52 SOURCE: Rabbit