CST, 49893SF, Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Integrin Beta2 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49893) is ready to ship. Product Usage Information This product is the carrier free version of product #72607. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 72607 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Integrin beta2 (E9O7W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total mouse Integrin β2 protein. This antibody does not cross-react with human Integrin β2 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser84 of mouse Integrin β2 protein. Background Integrins are transmembrane glycoproteins that form heterodimers consisting of one α and one β subunit. Integrin dimers act as receptors for extracellular matrix proteins and cell-surface ligands. Integrin signaling to (inside-out) and from (outside-in) extracellular molecules regulates multiple cellular processes, such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (1,2). Integrin β2 (CD18) is the β subunit of the leukocyte-specific integrin family. Leukocyte integrins include Integrin β2 (CD18)/αL (CD11a) (LFA-1, lymphocyte function associated antigen 1), Integrin β2 (CD18)/αM (CD11b) (Mac-1), Integrin β2 (CD18)/αX (CD11c), and Integrin β2 (CD18)/αD (CD11d). These integrins bind to immunoglobulin superfamily members, such as ICAM-1 and VCAM-1 on endothelial cells, to mediate firm adhesion and transendothelial migration of leukocytes (3). Integrin β2 (CD18) deficiency results in LAD (leukocyte adhesion deficiency), a disease characterized by impairment of leukocyte recruitment, resulting in an inability to fight infection (4). Alternate Names 2E6; AI528527; Cd18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; integrin beta 2; Integrin beta-2; ITB2; Itgb2; LAD; LCAMB; Lfa1; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta; MF17; OTTMUSP00000021575 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG