CST, 4997S, UBC3 Antibody

Polyclonal Antibody for studying UBC3B/UBC3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, UBC3 Antibody (CST #4997) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UBC3 Antibody detects endogenous levels of total UBC3 and UBC3B protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human UBC3. Antibodies are purified by protein A and peptide affinity chromatography. Background Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp) complex mediates degradation of b-catenin (6). Alternate Names (E3-independent) E2 ubiquitin-conjugating enzyme R1; CDC34; CDC34B; cell division cycle 34 homolog; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34, ubiqiutin conjugating enzyme; E2 ubiquitin-conjugating enzyme R1; E2 ubiquitin-conjugating enzyme R2; E2-CDC34; E2-CDC34B; FLJ20419; MGC10481; UB2R1; UB2R2; UBC3; UBC3B; UBCH3; UBE2R1; UBE2R2; ubiquitin carrier protein; Ubiquitin carrier protein R2; ubiquitin conjugating enzyme E2 R2; ubiquitin conjugating enzyme E2R 2; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B; Ubiquitin-protein ligase R1; Ubiquitin-protein ligase R2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit