CST, 50025S, PCGF5 (F5X4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PCGF5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PCGF5 (F5X4O) Rabbit Monoclonal Antibody (CST #50025) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PCGF5 (F5X4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total PCGF5 protein. This antibody does not cross-react with PCGF3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val9 of human PCGF5 protein. Background Polycomb group RING finger homologs (PCGF1-6) are essential proteins for the assembly of the polycomb repressive complex 1 (PRC1) group of complexes. RING1A/B and a unique PCGF protein make up the catalytic core of PRC1 to ubiquitinate histone H2A. Differences in binding specificities give PRC1 complexes unique functions depending on which PCGF is included (1-3). PCGF3 is closely related to PCGF5 and recruits PRC1 and PRC2 in response to Xist expression (4). PCGF3/5 can act as transcriptional activators in embryonic stem cells, where they interact with Tex10, a pluripotency and super-enhancer regulator (5). Alternate Names MGC16202; PCGF5; polycomb group ring finger 5; Polycomb group RING finger protein 5; ring finger protein (C3HC4 type) 159; RING finger protein 159; RNF159 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG