CST, 50033SF, IL-2R alpha/CD25 (E4B2Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IL2RA mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, IL-2R alpha/CD25 (E4B2Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #50033) is ready to ship. Product Usage Information This product is the carrier-free version of product #36128. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 36128 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IL-2R alpha/CD25 (E4B2Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IL-2Rα/CD25 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp131 of mouse IL-2Rα/CD25 protein. Background Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). Alternate Names CD25; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; Il2r; Il2ra; interleukin 2 receptor, alpha chain; Interleukin-2 receptor subunit alpha; Ly-4; Ly-43; MGC130561; OTTMUSP00000012002; p55; p55 chain Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-80 Source/Isotype: Rabbit IgG