CST, 50156T, RPRD1B (E8J9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RPRD1B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RPRD1B (E8J9U) Rabbit Monoclonal Antibody (CST #50156) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RPRD1B (E8J9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total RPRD1B protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RPRD1B protein. Background Regulation of nuclear pre-mRNA domain-containing 1B (RPRD1B), also known as cell cycle-related and expression-elevated protein in tumor (CREPT), is a transcriptional regulator that interacts with the POLR2A catalytic subunit of RNA polymerase II (Pol II) (1). It is also an oncoprotein that promotes cell proliferation and is upregulated in a variety of cancers, including gastric, colorectal, and non-small cell lung cancer (NSCLC) (2-4). RPRD1B contains a CTD-interacting domain (CID), which interacts with the C-terminal domain (CTD) of Pol II to regulate transcription termination (5). It is believed that this association enhances the binding of Pol II to the promoter, thereby increasing cyclin D1 transcription and promoting tumorigenesis (6). Specifically, RPRD1B promotes chromatin looping of the gene to recycle Pol II from the termination site to the promoter region (6). RPRD1B may also regulate the transcription of various genes through associations with STAT3 and the histone acetyltransferase p300 (7,8). Alternate Names C20orf77; Cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; CREPT; dJ1057B20.2; DKFZp434P0735; FLJ44520; K-H; Ku70-binding protein 5-Hera; Kub5-Hera; NET60; regulation of nuclear pre-mRNA domain containing 1B; Regulation of nuclear pre-mRNA domain-containing protein 1B; RPR1B; RPRD1B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG