CST, 50292S, TRIM29/ATDC (E1L4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATDC/TRIM29. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM29/ATDC (E1L4E) Rabbit Monoclonal Antibody (CST #50292) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TRIM29/ATDC (E1L4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM29/ATDC protein. This antibody also cross-reacts with an unidentified protein of 49 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln499 of human TRIM29/ATDC protein. Background Tripartite motif-containing protein 29 (TRIM29, ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. The TRIM29 protein binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3). Alternate Names Ataxia telangiectasia group D-associated protein; ataxia-telangiectasia group D-associated protein; ATDC; FLJ36085; TRI29; TRIM29; tripartite motif containing 29; tripartite motif protein TRIM29; tripartite motif-containing 29; Tripartite motif-containing protein 29 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG