CST, 5029S, MLK1 (D66E2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MLK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MLK1 (D66E2) Rabbit Monoclonal Antibody (CST #5029) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MLK1 (D66E2) Rabbit Monoclonal Antibody detects endogenous levels of total MLK1 protein. This antibody also cross-reacts with a 45 kDa protein of unknown origin. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1070 of human MLK1 protein. Background Mixed-lineage kinases (MLKs) belong to the mitogen activated kinase kinase kinase (MAPKKK) family of dual-specificity protein kinases. While not particularly well conserved at the sequence level, MLK1, 2 and 3 share a conserved domain structure consisting of a catalytic core and two isoleucine/leucine zipper motifs among other protein-protein binding domains (1). MLK1 preferentially stimulates the JNK (c-Jun amino-terminal kinase) pathway in response to agonists and stress (2). Although multiple phosphorylation events are required for full activation of MLK1, two autophosphorylation sites within the activation loop (Ser308 and Thr312) appear to be the predominant activation residues (3). In neuronal cells, MLK1 appears to function downstream of the small G-proteins Rac1 and Cdc42 and upstream of MKK4 and MKK7 to promote apoptosis (2). Alternate Names M3K9; MAP3K9; MEKK9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; mixed lineage kinase 1 (tyr and ser/thr specificity); MLK1; PRKE1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG