CST, 50308T, TAF6L (F3Q9O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TAF6L. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, TAF6L (F3Q9O) Rabbit Monoclonal Antibody (CST #50308) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity TAF6L (F3Q9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total TAF6L protein. This antibody detects a 250 kDa and a 19 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu598 of human TAF6L protein. Background TATA-box binding protein associated factor 6 like (TAF6L) and TAF5L are components of the SAGA complex, a histone acetyltransferase-containing complex that controls transcription by activation of target genes through histone acetylation (1). TAF6L and TAF5L are capable of activating c-Myc and Oct-4 and their downstream targets, controlling self-renewal in embryonic stem cells (2). TAF6L has been identified as a marker for glioma and bladder cancer (3,4). Alternate Names FLJ11136; MGC4288; p300/CBP-associated factor (PCAF)-associated factor 65; PAF65-alpha; PAF65A; PCAF-associated factor 65 alpha; PCAF-associated factor 65-alpha; TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa; TAF6L; TATA-box binding protein associated factor 6 like Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG