CST, 5030T, MEF2C (D80C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEF2C. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MEF2C (D80C1) Rabbit Monoclonal Antibody (CST #5030) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MEF2C (D80C1) Rabbit Monoclonal Antibody detects endogenous levels of total MEF2C protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Met182 of human MEF2C protein. Background MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, there are four MEF2C-related genes (MEF2A, MEF2B, MEF2C and MEF2D) that encode proteins that exhibit significant amino acid sequence similarity within their DNA binding domains and, to a lesser extent, throughout the rest of the proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Recently, several groups have reported MEF2 binding activity and MEF2 proteins in a wide variety of cell types where these proteins appear to play an important role in growth factor- and stress-induced early gene responses (4-6). Alternate Names C5DELq14.3; DEL5q14.3; MADS box transcription enhancer factor 2, polypeptide C; MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C); MEF2C; myocyte enhancer factor 2C; Myocyte-specific enhancer factor 2C Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50-60 Source/Isotype: Rabbit IgG