CST, 50311S, EPLIN (D1A7A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eplin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, EPLIN (D1A7A) Rabbit Monoclonal Antibody (CST #50311) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity EPLIN (D1A7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total EPLIN protein, including EPLIN-α and EPLIN-β isoforms. Based on the absence of signal in NCI-H28 cells, which appear to express low levels of β-Eplin, this antibody may have a preference for α-Eplin by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly455 of human EPLIN protein. Background Epithelial Protein Lost in Neoplasm (EPLIN) is an actin-binding protein that regulates actin filament dynamics and cross-linking (1). Alpha and beta isoforms are generated from alternate promoters, with the EPLIN-β isoform representing the full-length protein and the EPLIN-α isoform lacking the amino-terminal 160 amino acids (2). Increased expression of EPLIN protein results in more abundant and larger actin stress fibers due to stabilizing of cross-links and inhibition of actin depolymerization. EPLIN protein inhibits Rac1-promoted membrane ruffling and Arp2/3-associated actin filament branching (1). Research studies demonstrate reduced EPLIN-α expression in tumor tissues, and correlate this reduction with increased invasiveness and poor clinical outcomes (3). The EPLIN protein is an important negative regulator of the epithelial-mesenchymal transition (EMT)(4). While EMT is a critical process during normal embryonic development, dysregulation in transformed cells is a key step in the transition to metastasis (5). Alternate Names 1110021C24Rik; Epithelial protein lost in neoplasm; epithelial protein lost in neoplasm beta; EPLIN; FLJ38853; LDLCQ8; LIM domain and actin binding 1; LIM domain and actin-binding protein 1; LIMA1; MGC131726; SREBP3; sterol regulatory element binding protein 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 85, 110 Source/Isotype: Rabbit IgG