CST, 50318S, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying cre recombinase. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #50318) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes transfected and transgenic levels of total Cre recombinase protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein. Background Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). Alternate Names cre; cre recombinase; cyclization recombinase; Recombinase cre Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 37 Source/Isotype: Rabbit IgG