CST, 5031T, Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jak3 (Tyr980/Tyr981) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit Monoclonal Antibody (CST #5031) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit Monoclonal Antibody detects endogenous levels of Jak3 when phosphorylated at Tyr980/981. Some reactivity may exist with single phosphorylation at these sites. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Tyr980/981 of human and mouse Jak3 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common γ subunit (γc) and a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation is highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within its activation loop (12-14). Alternate Names JAK-3; JAK3; JAK3_HUMAN; JAKL; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L-JAK; Leukocyte janus kinase; LJAK; Tyrosine-protein kinase JAK3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit IgG