CST, 5034T, La Antigen (D19B3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LA antigen. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, La Antigen (D19B3) Rabbit Monoclonal Antibody (CST #5034) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity La Antigen (D19B3) Rabbit Monoclonal Antibody detects endogenous levels of total La Antigen protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu97 of human La Antigen protein. Background La antigen is recognized by antibodies in patients with autoimmune disorders such as systemic lupus erythematosus and Sjögren's syndrome (1). La antigen binds to the 5'-noncoding region of poliovirus RNA and is an IRES trans-acting factor (1,2). Depletion of La antigen reduces the function of poliovirus IRES (3). La antigen, when phosphorylated at Ser366, has been shown to associate with nuclear precursor tRNAs and facilitate their processing (4). The nonphosphorylated La antigen interacts with the mRNAs that have 5'-terminal oligopyrimidine (5'TOP) motifs to control protein synthesis (4). Alternate Names autoantigen La; La; La autoantigen; La ribonucleoprotein; La ribonucleoprotein domain family, member 3; La/SSB; LARP3; lupus La antigen; Lupus La protein; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B; Sjogren syndrome antigen B (autoantigen La); small RNA binding exonuclease protection factor La; SS-B; SS-B/La protein; SSB Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG