CST, 50402T, DDX1 (F5C8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX1 (F5C8A) Rabbit Monoclonal Antibody (CST #50402) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DDX1 (F5C8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX1 protein. This antibody detects a 52 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly686 of human DDX1 protein. Background Dead box protein 1 (DDX1) is a DEAD box RNA helicase that is involved in 3'-end processing of pre-mRNA. DDX1 is capable of binding to poly(A) RNA and interacts with several key RNA-binding proteins, including hnRNP K, and forms discrete nuclear foci with the CSTF complex called DDX1 bodies (1,2). During DNA damage, DDX1 is recruited to double-strand breaks (DSBs) upon phosphorylation by ATM, where it plays a role in RNA clearance at the DSB sites (3). DDX1 phosphorylation has also been shown to increase expression of a subset of microRNAs (miRNAs) that can inhibit tumor progression (4). DDX1 has been shown to bind to the HIV-1 Rev protein, and DDX1 downregulation disrupts viral production (5,6). Additionally, DDX1 has been implicated in other viral infections, including SARS-CoV-2, where DDX1 binds the nucleocapsid protein to inhibit its function (7). DDX1 has been described in various cancer types, but notably, the gene is co-amplified with in neuroblastoma (8-10). Alternate Names ATP-dependent RNA helicase DDX1; DBP-RB; DDX1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD-box helicase 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-box helicase 1; UKVH5d Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG