CST, 50423SF, VAMP2 (D6O1A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying VAMP2. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, VAMP2 (D6O1A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #50423) is ready to ship. Product Usage Information This product is the carrier free version of product #13508. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13508 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity VAMP2 (D6O1A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total VAMP2 protein. This antibody does not cross-react with VAMP1 protein and recognizes VAMP2 protein by IF-IC analysis in mouse and rat cells. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human VAMP2 protein. Background Vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin) is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium (2). In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release (2). In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils (3) and release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5). Alternate Names FLJ11460; NEDHAHM; SYB2; synaptobrevin 2; Synaptobrevin-2; VAMP-2; VAMP2; vesicle associated membrane protein 2; Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG