CST, 50490S, CTLA-4 (E5S7E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTLA-4 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CTLA-4 (E5S7E) Rabbit Monoclonal Antibody (CST #50490) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51435 . Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity CTLA-4 (E5S7E) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse CTLA-4 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse CTLA-4 protein. Background Cytotoxic T-lymphocyte protein 4 (CTLA-4, CD152) is an Ig superfamily member that negatively regulates early T cell activation (1-4). The CTLA-4 protein is primarily expressed on T cells, including CD8 cytotoxic T cells, CD4 helper T cells, and CD4 /FoxP3 regulatory T cells (1,2). CTLA-4 protein competes with CD28 for B7.1 (CD80) and B7.2 (CD86) binding at the cell surface, which results in the downregulation of T cell activity (5). The activation of SHP-2 and PP2A downstream of CTLA-4 attenuates TCR signaling (6). Research studies indicate that knockout mice display lymphoproliferative disorders leading to early death, confirming the role of CTLA-4 as a negative regulator of T cells (7). Mutations in the corresponding gene are associated with multiple disorders, including insulin-dependent diabetes mellitus, Graves' disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, and type V autoimmune lymphoproliferative syndrome (8,9). Additional studies demonstrate that CTLA-4 blockade is an effective strategy for tumor immunotherapy (10-12). Alternate Names Cd152; CD152 antigen; Ctla; CTLA-4; Ctla4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; Ly-5; Ly-56; OTTMUSP00000002168 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG