CST, 50535T, Phospho-PLC gamma2 (Tyr759) (E9E9Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PLCg2 (Tyr759) phosphate. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PLC gamma2 (Tyr759) (E9E9Y) Rabbit Monoclonal Antibody (CST #50535) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PLC gamma2 (Tyr759) (E9E9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of PLCγ2 protein only when phosphorylated at Tyr759. This antibody may cross-react with other tyrosine-phosphorylated proteins like EGFR. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr759 of human PLCγ2 protein. Background Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP ) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP ) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). Alternate Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; APLAID; FCAS3; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; Phospholipase C-gamma-2; Phospholipase C-IV; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); PLC-gamma-2; PLC-IV; PLCG2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG