CST, 5053T, Argonaute 1 (D84G10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ago1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Argonaute 1 (D84G10) Rabbit Monoclonal Antibody (CST #5053) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Argonaute 1 (D84G10) Rabbit Monoclonal Antibody detects endogenous levels of total Argonaute 1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein. Background Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. miRNAs are about 21 nucleotides in length and have been implicated in many cellular processes, such as development, differentiation, and stress response (1,2). miRNAs regulate gene expression by modulating mRNA translation or stability (2). miRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family, and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of miRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1). Alternate Names AGO1; argonaute 1; argonaute 1, RISC catalytic component; Argonaute RISC catalytic component 1; argonaute RISC component 1; Argonaute1; DKFZp686M13167; eIF-2C 1; EIF2C; eIF2C 1; EIF2C1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; GERP95; Golgi Endoplasmic Reticulum protein 95 kDa; hAgo1; Protein argonaute-1; Putative RNA-binding protein Q99; Q99 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG