CST, 50546T, LRRC15 (E4X8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LRRC15. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LRRC15 (E4X8J) Rabbit Monoclonal Antibody (CST #50546) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 52305 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity LRRC15 (E4X8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total LRRC15 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu100 of human LRRC15 protein. Background LRRC15 (Leucine-rich repeat-containing protein 15, LIB) is a transmembrane protein belonging to the LRR family of proteins involved in cell-cell and cell-ECM (extracellular matrix) interactions (1-4). It has been identified as a marker for TGF-β-driven CAFs (cancer-associated fibroblasts), which are associated with immunotherapy resistance (3,4). LRRC15 is highly expressed in multiple solid tumor types, with low expression in normal tissues, making it a good therapeutic candidate for cancers with high stromal content, such as pancreatic cancer, non-small cell lung cancer (NSCLC), and triple-negative breast cancer (3,4). Alternate Names hLib; leucine rich repeat containing 15; leucine-rich repeat protein induced by beta amyloid; Leucine-rich repeat protein induced by beta-amyloid homolog; Leucine-rich repeat-containing protein 15; LIB; LRC15; LRRC15 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80-100 Source/Isotype: Rabbit IgG