CST, 50584T, MG-H1 Glycated Histone H3 (Arg17) (E2W7I) Mouse Monoclonal Antibody

Monoclonal Antibody for studying H3 (Arg17) glycation. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MG-H1 Glycated Histone H3 (Arg17) (E2W7I) Mouse Monoclonal Antibody (CST #50584) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the N-terminal tail of histone H3 in which Arg17 is MG-H1 glycated. Background Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, composed of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). Glycation is an additional posttranslational modification that occurs non-enzymatically within cells, involving the condensation of monosaccharides or glycolytic by-products with lysine or arginine residues (7). Methylglyoxal (MGO), a highly reactive by-product of glycolysis, readily binds with arginine to form the product methylglyoxal-derived hydroimidazolone (MG-H) in three possible isomeric forms: MG-H1, MG-H2, and MG-H3 (8). The accumulation of MGO-glycated proteins is readily observed in aging cells, individuals with hyperglycemia or diabetes, and various types of cancer (9,10). Notably, MGO-glycated histone H3 has been shown to accumulate in breast cancer cells and patient tumors, potentially contributing to genomic instability by modifying chromatin structure (11). Alternate Names H3; H3 clustered histone 1; H3 histone family, member A; H3/A; H31; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; Histone H3; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Mouse IgG2b kappa