CST, 5058S, NPC1L1 Antibody

Polyclonal Antibody for studying NPC1L1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NPC1L1 Antibody (CST #5058) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NPC1L1 Antibody detects endogenous levels of total NPC1L1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human NPC1L1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Niemann-Pick C1-like 1 (NPC1L1) is a transmembrane protein that plays a critical role in cholesterol absorption (1). It is highly expressed in small intestine and localized along the brush border in both human and mouse epithelial cells (2,3). NPC1L1 mediates cholesterol uptake via vesicular endocytosis (4). Ezetimibe, a potent cholesterol absorption inhibitor used to treat hypercholesterolemia (5), inhibits cholesterol uptake by preventing NPC1L1 from incorporating into clathrin-coated vesicles (4). Alternate Names LDLCQ7; Niemann-Pick C1-like protein 1; NPC1 (Niemann-Pick disease, type C1, gene)-like 1; NPC1 like 1; NPC1 like intracellular cholesterol transporter 1; NPC1-like intracellular cholesterol transporter 1; NPC11L1; NPC1L1; NPCL1; SLC65A2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit