CST, 50616S, SGO2 (E9K4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Sgo2. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SGO2 (E9K4N) Rabbit Monoclonal Antibody (CST #50616) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84360 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SGO2 (E9K4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SGO2 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize isoforms 1-3 of human SGO2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly15 of human SGO2 protein. Background SGO2 (Shugoshin-2, SGOL2) is a centromeric protein required for the protection of cohesion complexes in meiosis (1). In mitosis, SGO2 and MAD2 regulate separase activity in the absence of securin to ensure appropriate timing of chromosome segregation (2). SGO2 is highly expressed in prostate cancer, and it promotes proliferation and migration by stabilizing the small GTPase Rab1A (3). High SGO2 expression in hepatocellular carcinoma (HCC) predicts poor overall survival (4), promoting tumor proliferation by regulating MAD2 expression and function (5). Alternate Names FLJ25211; MGC129665; Sgo2; SGOL2; Shugoshin 2; Shugoshin-2; Shugoshin-like 2; shugoshin-like 2 (S. pombe); Tripin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG