CST, 5061S, HO-1 (P249) Antibody

Polyclonal Antibody for studying HO-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, HO-1 (P249) Antibody (CST #5061) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HO-1 (P249) Antibody detects endogenous levels of total HO-1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Pro249 of human HO-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory, and cytoprotective functions (2). HO comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer's disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5). Alternate Names bK286B10; heat shock protein, 32-kD; heme oxygenase (decycling) 1; Heme oxygenase 1; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit