CST, 50703S, RBM4 Antibody

Polyclonal Antibody for studying RBM4. Validated for Western Blotting. Highly specific and rigorously validated in-house, RBM4 Antibody (CST #50703) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RBM4 Antibody recognizes endogenous levels of total RBM4 protein. This antibody also detects RBM4B protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro317 of human RBM4 protein. Antibodies are purified by peptide affinity chromatography. Background RBM4 functions as an RNA-binding protein and precursor mRNA splicing factor that antagonizes activity of PTBP1-mediated splicing (1-4). Upon phosphorylation at Ser309, RBM4 accumulates in stress granules and suppresses cap-dependent translation through eIF4A (3). Exon skipping of MEF2C by RBM4 has been shown to play a key role in the differentiation of brown adipocytes (5). The splicing activities of RBM4 seem to suppress tumorigenesis in the context of multiple cancer types (5-8). Alternate Names DKFZp547K0918; hLark; LARK; Lark homolog; MGC75138; RBM4; RBM4A; RNA binding motif protein 4; RNA-binding motif protein 4; RNA-binding motif protein 4a; RNA-binding protein 4; transcriptional coactivator CoAZ; ZCCHC21; ZCRB3A; zinc finger CCHC-type and RNA binding motif 3A Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 41 SOURCE: Rabbit