CST, 5071S, ELP1/IKBKAP Antibody

Polyclonal Antibody for studying IKBKAP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ELP1/IKBKAP Antibody (CST #5071) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ELP1/IKBKAP Antibody recognizes endogenous levels of total ELP1/IKBKAP protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELP1/IKBKAP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6). The ELP1/IKBKAP subunit of Elongator was initially thought to function as a scaffolding protein within the NFκB signaling pathway (7). It contains several WD40 domains and is critical for the formation of the Elongator complex (8). Investigators have determined that mutations in ELP1 are the cause of Familial Dysautonomia (FD), an autosomal recessive neurodegenerative disorder (9). Research studies have demonstrated that defects in Elongator function upon ELP1 mutation affect transcription elongation of several genes involved in cell motility and neuronal development that may be the underlying cause of the neuropathology of FD patients (10,11). Alternate Names DKFZp781H1425; DYS; elongator acetyltransferase complex subunit 1; Elongator complex protein 1; ELP1; FD; FLJ12497; IKAP; IkappaB kinase complex-associated protein; IKBKAP; IKI3; IKK complex-associated protein; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein; p150; TOT1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit