CST, 50830T, GHF-1 (F9C3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GHF-1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, GHF-1 (F9C3D) Rabbit Monoclonal Antibody (CST #50830) is ready to ship. Product Usage Information For optimal ChIP, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity GHF-1 (F9C3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total GHF-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu86 of human GHF-1 protein. Background Growth hormone factor 1 (GHF-1), also known as Pit-1, is a POU domain transcription factor essential for the development and function of specific anterior pituitary cells (i.e., somatotrophs, lactotrophs, and thyrotrophs) (2). It activates the genes for growth hormone, prolactin, and TSH beta, crucial for hormone production and pituitary cell lineage determination (1,2). Mutations in (the gene encoding GHF-1) are a major cause of combined pituitary hormone deficiency and isolated growth hormone deficiency, leading to impaired hormone secretion and developmental issues (3). While GHF-1 is crucial for normal pituitary development, its role in pituitary tumorigenesis is complex and not fully elucidated. Some pituitary adenomas, particularly those of the somatotroph and lactotroph lineages, express GHF-1. However, mutations are not a common cause of sporadic pituitary adenomas. Aberrant GHF-1 expression or activity might contribute to tumor progression in some cases, but further research is needed (4,5). Alternate Names CPHD1; GHF-1; GHF1; Growth hormone factor 1; PIT-1; PIT1; pituitary transcript factor 1; Pituitary-specific positive transcription factor 1; pituitary-specific transcription factor 1; POU class 1 homeobox 1; POU domain, class 1, transcription factor 1; POU1F1; POU1F1a Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG