CST, 50832T, Shc (E6I4B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Shc1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Shc (E6I4B) Rabbit Monoclonal Antibody (CST #50832) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Shc (E6I4B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Shc protein. This antibody detects three isoforms at 46, 52, and 66 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human SHC1 protein. Background SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4). Alternate Names FLJ26504; SH2 domain protein C1; SHC; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC adaptor protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; SHC1; SHCA; Src homology 2 domain-containing-transforming protein C1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46, 52, 66 Source/Isotype: Rabbit IgG