CST, 50844S, MEX3C (D5T2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEX3C. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MEX3C (D5T2Z) Rabbit Monoclonal Antibody (CST #50844) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MEX3C (D5T2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total MEX3C protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala54 of human MEX3C protein. Background MEX3C is an RNA-binding E3 ubiquitin ligase implicated in a diverse set of biological functions. Along with PIGN and ZNF516, MEX3C has been identified as a suppressor of chromosome instability (CIN), which drives intratumor heterogeneity and has been associated with poor prognosis in colon cancer. Silencing of the gene induces chromosome instability through replication stress/impaired replication fork progression (1). In mice, absence of the gene causes growth retardation and reduced insulin-like growth factor 1 (IGF1)Â expression (2). Researchers have shown that murine MEX3C plays a role in controlling energy expenditure, physical activity and degree of adiposity (3,4). In the immune system, MEX3C is involved in post-transcriptional regulation of HLA-A2 (5), and plays a role in eliciting antiviral response (6). Alternate Names BM-013; FLJ38871; mex-3 homolog C (C. elegans); mex-3 RNA binding family member C; MEX-3C; MEX3C; MEX3C variant 1; MEX3C variant 2; ring finger and KH domain containing 2; RING finger and KH domain-containing protein 2; RING finger protein 194; RING-type E3 ubiquitin transferase MEX3C; RKHD2; RNA-binding E3 ubiquitin-protein ligase MEX3C; RNA-binding protein MEX3C; RNF194 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG