CST, 50845SF, Atg7 (F4V2N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATG7. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Atg7 (F4V2N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #50845) is ready to ship. Product Usage Information This product is the carrier free version of product #88577. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 88577 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Atg7 (F4V2N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Atg7 protein. Based on epitope sequence, this antibody is predicted to detect all isoforms of Atg7 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys45 of human Atg7 protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related ( ) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Alternate Names APG7 autophagy 7-like; APG7-like; APG7L; ATG12-activating enzyme E1 ATG7; ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); autophagy related 7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG