CST, 50901T, SRSF2 (F1P3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SFRS2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SRSF2 (F1P3N) Rabbit Monoclonal Antibody (CST #50901) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SRSF2 (F1P3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRSF2 protein. This antibody detects a 22 kDa protein and a 140 kDa protein of unknown identities in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser101 of human SRSF2 protein. Background Serine/arginine-rich splicing factor 2 (SRSF2/SFRS2), also known as splicing factor SC35, is a well-studied serine/arginine-rich splicing factor (SR) protein. SR proteins are crucial regulators in precursor RNA splicing, a process associated with maintaining genome stability, regulating mRNA export and stability, and translation (1,2). SRSF2 and other SR proteins harbor an N-terminal RNA recognition motif (RRM) that is involved in sequence-specific RNA binding and a C-terminal arginine/serine-rich (RS) domain that facilitates interactions with other splicing proteins (2-5). Similar to most other SR proteins, SRSF2 is essential for normal development as its germline deletion is embryonically lethal in mice (6,7). Moreover, mutations in the gene are often found in myelodysplasia, with the most common hotspot mutation, a substitution of proline for histidine at position 95, causing altered recognition of exonic splicing enhancers and aberrantly spliced transcripts (8-10). In myeloid malignancies, mutations have been linked to abnormal differentiation of hematopoietic stem and progenitor cells (11). Alternate Names PR264; Protein PR264; SC-35; SC35; serine and arginine rich splicing factor 2; Serine/arginine-rich splicing factor 2; SFRS2; SFRS2A; Splicing component, 35 kDa; Splicing factor SC35; Splicing factor, arginine/serine-rich 2; SR splicing factor 2; SRp30b; SRSF2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG